Regulatory

Part:BBa_J58011:Design

Designed by: Caterina Mata Garcia   Group: iGEM06_Valencia   (2006-10-23)


Promoter which is activated by cI and CRP, using a transcription logic function type AND


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We take the lambda Prm promoter sequence from the Registry, and we add the CRP binding site upstream of that promoter. The centered position of the cI operator is -42, and for the CRP is -93,5.

Source

The sources of this promoter are, on the one hand, from the bacteriophage lambda, and, on the other hand, from the E. coli. We take the lambda Prm promoter (modified to be activated but not repressed by cI, part ref. BBa_I12007), and the binding site region for CRP from E. coli.

References

JK Joung, DM Koepp, A Hochschild. Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein. Science (1994), 265, 1863-1866.